Dr Joshua Hamey

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Joshua Hamey is a post-doctoral Research Associate in the School of Biotechnology and Biomolecular Sciences in the group of Professor Marc Wilkins. His research focuses on understanding proteins and their post-translational modifications, particularly through use of mass spectrometry.

Phone

+61 2 9348 1312

E-mail

j.hamey@unsw.edu.au

Publications
Grants
Awards

Journal articles | 2025

Hamey JJ; Shah M; Wade JD; Bartolec TK; Wettenhall REH; Quinlan KGR; Williamson NA; Wilkins MR, 2025, 'SMYD5 is a ribosomal methyltransferase that trimethylates RPL40 lysine 22 through recognition of a KXY motif', Cell Reports, 44, http://dx.doi.org/10.1016/j.celrep.2025.115518

Journal articles | 2024

Hamey JJ; Nguyen A; Haddad M; Vázquez-Campos X; Pfeiffer PG; Wilkins MR, 2024, 'Methylation of elongation factor 1A by yeast Efm4 or human eEF1A-KMT2 involves a beta-hairpin recognition motif and crosstalks with phosphorylation', Journal of Biological Chemistry, 300, http://dx.doi.org/10.1016/j.jbc.2024.105639

Journal articles | 2024

Karakatsanis NM; Hamey JJ; Wilkins MR, 2024, 'Taking Me away: the function of phosphorylation on histone lysine demethylases', Trends in Biochemical Sciences, 49, pp. 257 - 276, http://dx.doi.org/10.1016/j.tibs.2023.12.004

Journal articles | 2024

Separovich RJ; Karakatsanis NM; Gao K; Fuh D; Hamey JJ; Wilkins MR, 2024, 'Proline-directed yeast and human MAP kinases phosphorylate the Dot1p/DOT1L histone H3K79 methyltransferase', FEBS Journal, 291, pp. 2590 - 2614, http://dx.doi.org/10.1111/febs.17062

Journal articles | 2024

Smolnikov A; Padoani D; Jurczyluk J; Su Z; Hamey J; Wilkins M; Yuen M; Oates E, 2024, '492P Mass spectrometry as a technique for robust quantification of titin and other large muscle disease-associated proteins', Neuromuscular Disorders, 43, pp. 104441.329 - 104441.329, http://dx.doi.org/10.1016/j.nmd.2024.07.338

Journal articles | 2023

Hamey JJ; Wilkins MR, 2023, 'The protein methylation network in yeast: A landmark in completeness for a eukaryotic post-translational modification', Proceedings of the National Academy of Sciences of the United States of America, 120, http://dx.doi.org/10.1073/pnas.2215431120

Journal articles | 2022

Bartolec TK; Hamey JJ; Keller A; Chavez JD; Bruce JE; Wilkins MR, 2022, 'Differential Proteome and Interactome Analysis Reveal the Basis of Pleiotropy Associated With the Histidine Methyltransferase Hpm1p', Molecular and Cellular Proteomics, 21, http://dx.doi.org/10.1016/j.mcpro.2022.100249

Journal articles | 2022

Separovich RJ; Wong MWM; Bartolec TK; Hamey JJ; Wilkins MR, 2022, 'Site-specific Phosphorylation of Histone H3K36 Methyltransferase Set2p and Demethylase Jhd1p is Required for Stress Responses in Saccharomyces cerevisiae', Journal of Molecular Biology, 434, pp. 167500, http://dx.doi.org/10.1016/j.jmb.2022.167500

Journal articles | 2022

Tay AP; Hamey JJ; Martyn GE; Wilson LOW; Wilkins MR, 2022, 'Identification of Protein Isoforms Using Reference Databases Built from Long and Short Read RNA-Sequencing', Journal of Proteome Research, 21, pp. 1628 - 1639, http://dx.doi.org/10.1021/acs.jproteome.1c00968

Journal articles | 2021

Emery-Corbin SJ; Hamey JJ; Balan B; Rojas-López L; Svärd SG; Jex AR, 2021, 'Eukaryote-conserved histone post-translational modification landscape in Giardia duodenalis revealed by mass spectrometry', International Journal for Parasitology, 51, pp. 225 - 239, http://dx.doi.org/10.1016/j.ijpara.2020.09.006

Journal articles | 2021

Hamey JJ; Nguyen A; Wilkins MR, 2021, 'Discovery of Arginine Methylation, Phosphorylation, and Their Co-occurrence in Condensate-Associated Proteins in Saccharomyces cerevisiae', Journal of Proteome Research, 20, pp. 2420 - 2434, http://dx.doi.org/10.1021/acs.jproteome.0c00927

Journal articles | 2021

Hamey JJ; Rakow S; Bouchard C; Senst JM; Kolb P; Bauer UM; Wilkins MR; Hart-Smith G, 2021, 'Systematic investigation of PRMT6 substrate recognition reveals broad specificity with a preference for an RG motif or basic and bulky residues', FEBS Journal, 288, pp. 5668 - 5691, http://dx.doi.org/10.1111/febs.15837

Journal articles | 2021

Separovich RJ; Wong MWM; Chapman TR; Slavich E; Hamey JJ; Wilkins MR, 2021, 'Post-translational modification analysis of Saccharomyces cerevisiae histone methylation enzymes reveals phosphorylation sites of regulatory potential', Journal of Biological Chemistry, 296, http://dx.doi.org/10.1074/JBC.RA120.015995

Journal articles | 2020

Bartolec TK; Smith DL; Pang CNI; Xu YD; Hamey JJ; Wilkins MR, 2020, 'Cross-linking Mass Spectrometry Analysis of the Yeast Nucleus Reveals Extensive Protein-Protein Interactions Not Detected by Systematic Two-Hybrid or Affinity Purification-Mass Spectrometry', Analytical Chemistry, 92, pp. 1874 - 1882, http://dx.doi.org/10.1021/acs.analchem.9b03975

Journal articles | 2020

Emery-Corbin SJ; Hamey JJ; Ansell BRE; Balan B; Tichkule S; Stroehlein AJ; Cooper C; McInerney BV; Hediyeh-Zadeh S; Vuong D; Crombie A; Lacey E; Davis MJ; Wilkins MR; Bahlo M; Svard SG; Gasser RB; Jex AR, 2020, 'Eukaryote-conserved methylarginine is absent in diplomonads and functionally compensated in Giardia', Molecular Biology and Evolution, 37, pp. 3525 - 3549, http://dx.doi.org/10.1093/molbev/msaa186

Journal articles | 2020

Pang CNI; Ballouz S; Weissberger D; Thibaut LM; Hamey JJ; Gillis J; Wilkins MR; Hart-Smith G, 2020, 'Analytical Guidelines for co-fractionation Mass Spectrometry Obtained through Global Profiling of Gold Standard Saccharomyces cerevisiae Protein Complexes', Molecular and Cellular Proteomics, 19, pp. 1876 - 1895, http://dx.doi.org/10.1074/mcp.RA120.002154

Journal articles | 2020

Separovich RJ; Wong MWM; Chapman TR; Slavich E; Hamey JJ; Wilkins MR, 2020, 'Post-translational modification analysis of Saccharomyces cerevisiae histone methylation enzymes reveals phosphorylation sites of regulatory potential.', J Biol Chem, pp. 100192, http://dx.doi.org/10.1074/jbc.RA120.015995

Journal articles | 2020

Smith DL; Erce MA; Lai YW; Tomasetig F; Hart-Smith G; Hamey JJ; Wilkins MR, 2020, 'Crosstalk of Phosphorylation and Arginine Methylation in Disordered SRGG Repeats of Saccharomyces cerevisiae Fibrillarin and Its Association with Nucleolar Localization', Journal of Molecular Biology, 432, pp. 448 - 466, http://dx.doi.org/10.1016/j.jmb.2019.11.006

Journal articles | 2019

Lowe BR; Maxham LA; Hamey JJ; Wilkins MR; Partridge JF, 2019, 'Histone H3 mutations: An updated view of their role in chromatin deregulation and cancer', Cancers, 11, pp. 1 - 24, http://dx.doi.org/10.3390/cancers11050660

Journal articles | 2019

Tay AP; Liang A; Hamey JJ; Hart-Smith G; Wilkins MR, 2019, 'MS2-Deisotoper: A Tool for Deisotoping High-Resolution MS/MS Spectra in Normal and Heavy Isotope-Labelled Samples', Proteomics, 19, pp. e1800444, http://dx.doi.org/10.1002/pmic.201800444

Journal articles | 2018

Chia SZ; Lai YW; Yagoub D; Lev S; Hamey JJ; Ignatius Pang CN; Desmarini D; Chen Z; Djordjevic JT; Erce MA; Hart-Smith G; Wilkins MR; Pang I, 2018, 'Knockout of the Hmt1p arginine methyltransferase in saccharomyces cerevisiae leads to the dysregulation of phosphate-associated genes and processes', Molecular and Cellular Proteomics, 17, pp. 2462 - 2479, http://dx.doi.org/10.1074/mcp.RA117.000214

Journal articles | 2018

Hamey JJ; Separovich RJ; Wilkins MR, 2018, 'MT-MAMS: Protein Methyltransferase Motif Analysis by Mass Spectrometry', Journal of Proteome Research, 17, pp. 3485 - 3491, http://dx.doi.org/10.1021/acs.jproteome.8b00396

Journal articles | 2018

Hamey JJ; Wilkins MR, 2018, 'Methylation of Elongation Factor 1A: Where, Who, and Why?', Trends in Biochemical Sciences, 43, pp. 211 - 223, http://dx.doi.org/10.1016/j.tibs.2018.01.004

Journal articles | 2017

Hamey JJ; Wienert B; Quinlan KGR; Wilkins MR, 2017, 'METTL21B Is a Novel Human lysine methyltransferase of translation elongation factor 1A: Discovery by CRISPR/Cas9 Knockout', Molecular and Cellular Proteomics, 16, pp. 2229 - 2242, http://dx.doi.org/10.1074/mcp.M116.066308

Journal articles | 2016

Hamey JJ; Hart-Smith G; Erce MA; Wilkins MR, 2016, 'The activity of a yeast Family 16 methyltransferase, Efm2, is affected by a conserved tryptophan and its N-terminal region', FEBS Open Bio, 6, pp. 1320 - 1330, http://dx.doi.org/10.1002/2211-5463.12153

Journal articles | 2016

Hamey JJ; Winter DL; Yagoub D; Overall CM; Hart-Smith G; Wilkins MR, 2016, 'Novel N-terminal and lysine methyltransferases that target translation elongation factor 1A in yeast and human', Molecular and Cellular Proteomics, 15, pp. 164 - 176, http://dx.doi.org/10.1074/mcp.M115.052449

Journal articles | 2015

Yagoub D; Tay AP; Chen Z; Hamey JJ; Cai C; Chia SZ; Hart-Smith G; Wilkins MR, 2015, 'Proteogenomic Discovery of a Small, Novel Protein in Yeast Reveals a Strategy for the Detection of Unannotated Short Open Reading Frames', Journal of Proteome Research, 14, pp. 5038 - 5047, http://dx.doi.org/10.1021/acs.jproteome.5b00734

Journal articles | 2014

Zhang L; Hamey JJ; Hart-Smith G; Erce MA; Wilkins MR, 2014, 'Elongation factor methyltransferase 3 - A novel eukaryotic lysine methyltransferase', Biochemical and Biophysical Research Communications, 451, pp. 229 - 234, http://dx.doi.org/10.1016/j.bbrc.2014.07.110
Preprints | 2024

Hamey JJ; Shah M; Wade JD; Bartolec TK; Wettenhall REH; Quinlan KGR; Williamson NA; Wilkins MR, 2024, SMYD5 is a ribosomal methyltransferase which trimethylates RPL40 lysine 22 through recognition of a KXY motif, http://dx.doi.org/10.1101/2024.10.10.616381

2021: School of Biotechnology and Biomolecular Sciences (BABS) Early Career Researcher Grant
2020: UNSW Science Early Career Academic Network (ECAN) Seed Grant 2020

2021: The Australasian Proteomics Society Ken Mitchelhill Young Investigator Award
2019: Human Proteome Organisation ECR Travel award
2017: Students of the APS Presentation Award (Lorne Proteomics Symposium)
2015: The Sydney Protein Group Lorne Travel Scholarship
2015: Lorne Proteomics Symposium Poster Prize

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