Associate Professor Emma Sierecki

Associate Professor Emma Sierecki

Associate Professor
Medicine & Health
School of Biomedical Sciences

I obtained a PhD from the School of Pharmacy of Universite Rene Descartes in Paris, France, in a medicinal chemistry laboratory. I then undertook a postdoctoral training in the Pharmacology Department of the University of California San Diego (UCSD), where I developed skills in Biochemistry and Cell biology, with the idea of better understanding the drug targets. I combined my knowledge of chemistry and biology to conduct a drug discovery campaign that led to the identification of first-in-class inhibitors of the phosphatase PHLPP. Upon moving to Australia, in the Institute for Molecular Biology (IMB) at University of Queensland (UQ), I became interested in protein-protein interactions and developed the platform of assays to interrogate protein-protein interactions that is now at the heart of our work in the laboratory. Since joining UNSW in 2015, my group has worked on protein aggregation in neurodegenerative diseases and drug ability of transcription factors. My work is now focusing on understanding how protein-protein interactions are affected during pathological processes. The main axes of the laboratory are the study of the processes of protein aggregation and the development of a diagnostic test of Parkinson’s disease .

Location

Single Molecule Science C25, Level 3 Lowy Cancer Research Centre

  • Book Chapters | 2016
    O Carroll A; Moustaqil M; Leitao ADG; Gambin Y; Sierecki E, 2016, 'Protein self-interaction in health and disease: How dimerization, oligomerization and polymerization modulate protein function', in Protein-Protein Interactions (PPIs): Types, Methods for Detection and Analysis, pp. 97 - 151
  • Journal articles | 2024
    Akefe IO; Saber SH; Matthews B; Venkatesh BG; Gormal RS; Blackmore DG; Alexander S; Sieriecki E; Gambin Y; Bertran-Gonzalez J; Vitale N; Humeau Y; Gaudin A; Ellis SA; Michaels AA; Xue M; Cravatt B; Joensuu M; Wallis TP; Meunier FA, 2024, 'The DDHD2-STXBP1 interaction mediates long-term memory via generation of saturated free fatty acids', EMBO Journal, 43, pp. 533 - 567, http://dx.doi.org/10.1038/s44318-024-00030-7
    Journal articles | 2024
    Dickson CF; Hertel S; Tuckwell AJ; Li N; Ruan J; Al-Izzi SC; Ariotti N; Sierecki E; Gambin Y; Morris RG; Towers GJ; Böcking T; Jacques DA, 2024, 'The HIV capsid mimics karyopherin engagement of FG-nucleoporins', Nature, 626, pp. 836 - 842, http://dx.doi.org/10.1038/s41586-023-06969-7
    Journal articles | 2024
    Kambanis L; Ayoub A; Bedding MJ; Egelund PHG; Maxwell JWC; Franck C; Lambrechts L; Hawkins PME; Chisholm TS; Mackay JP; Sierecki E; Gambin Y; Kulkarni SS; Payne RJ, 2024, 'Expressed Protein Ligation in Flow', Journal of the American Chemical Society, 146, pp. 22027 - 22035, http://dx.doi.org/10.1021/jacs.4c07462
    Journal articles | 2024
    Lau D; Tang Y; Kenche V; Copie T; Kempe D; Jary E; Graves NJ; Biro M; Masters CL; Dzamko N; Gambin Y; Sierecki E, 2024, 'Single-Molecule Fingerprinting Reveals Different Growth Mechanisms in Seed Amplification Assays for Different Polymorphs of α-Synuclein Fibrils', ACS Chemical Neuroscience, http://dx.doi.org/10.1021/acschemneuro.4c00185
    Journal articles | 2023
    Chiang IKN; Graus MS; Kirschnick N; Davidson T; Luu W; Harwood R; Jiang K; Li B; Wong YY; Moustaqil M; Lesieur E; Skoczylas R; Kouskoff V; Kazenwadel J; Arriola-Martinez L; Sierecki E; Gambin Y; Alitalo K; Kiefer F; Harvey NL; Francois M, 2023, 'The blood vasculature instructs lymphatic patterning in a SOX7-dependent manner', EMBO Journal, 42, http://dx.doi.org/10.15252/embj.2021109032
    Journal articles | 2023
    Graves NJ; Gambin Y; Sierecki E, 2023, 'α-Synuclein Strains and Their Relevance to Parkinson’s Disease, Multiple System Atrophy, and Dementia with Lewy Bodies', International Journal of Molecular Sciences, 24, http://dx.doi.org/10.3390/ijms241512134
    Journal articles | 2023
    Underwood A; Rasicci DT; Hinds D; Mitchell JT; Zieba JK; Mills J; Arnold NE; Cook TW; Moustaqil M; Gambin Y; Sierecki E; Fontaine F; Vanderweele S; Das AS; Cvammen W; Sirpilla O; Soehnlen X; Bricker K; Alokaili M; Green M; Heeringa S; Wilstermann AM; Freeland TM; Qutob D; Milsted A; Jauch R; Triche TJ; Krawczyk CM; Bupp CP; Rajasekaran S; Francois M; Prokop JW, 2023, 'Evolutionary Landscape of SOX Genes to Inform Genotype-to-Phenotype Relationships', Genes, 14, http://dx.doi.org/10.3390/genes14010222
    Journal articles | 2022
    Baker MODG; Shanmugam N; Pham CLL; Ball SR; Sierecki E; Gambin Y; Steain M; Sunde M, 2022, 'The RHIM of the Immune Adaptor Protein TRIF Forms Hybrid Amyloids with Other Necroptosis-Associated Proteins', Molecules, 27, http://dx.doi.org/10.3390/molecules27113382
    Journal articles | 2022
    Lau D; Magnan C; Hill K; Cooper A; Gambin Y; Sierecki E, 2022, 'Single Molecule Fingerprinting Reveals Different Amplification Properties of α-Synuclein Oligomers and Preformed Fibrils in Seeding Assay', ACS Chemical Neuroscience, 13, pp. 883 - 896, http://dx.doi.org/10.1021/acschemneuro.1c00553
    Journal articles | 2021
    Bhumkar A; Magnan C; Lau D; Jun ESW; Dzamko N; Gambin Y; Sierecki E, 2021, 'Single-Molecule Counting Coupled to Rapid Amplification Enables Detection of α-Synuclein Aggregates in Cerebrospinal Fluid of Parkinson's Disease Patients', Angewandte Chemie - International Edition, 60, pp. 11874 - 11883, http://dx.doi.org/10.1002/anie.202014898
    Journal articles | 2021
    Bhumkar A; Magnan C; Lau D; Jun ESW; Dzamko N; Gambin Y; Sierecki E, 2021, 'Single‐Molecule Counting Coupled to Rapid Amplification Enables Detection of α‐Synuclein Aggregates in Cerebrospinal Fluid of Parkinson's Disease Patients', Angewandte Chemie, 133, pp. 11981 - 11990, http://dx.doi.org/10.1002/ange.202014898
    Journal articles | 2021
    Böcking T; Jacques DA; Lau D; Walsh JC; Dickson CF; Tuckwell A; Stear JH; Hunter DJB; Bhumkar A; Shah V; Turville SG; Sierecki E; Gambin Y, 2021, 'Rapid HIV-1 capsid interaction screening using fluorescence fluctuation spectroscopy', Analytical Chemistry, 93, pp. 3786 - 3793, http://dx.doi.org/10.1021/acs.analchem.0c04250
    Journal articles | 2021
    Clabbers MTB; Holmes S; Muusse TW; Vajjhala P; Thygesen SJ; Malde AK; Hunter DJB; Croll TI; Flueckiger L; Nanson JD; Rahaman MH; Aquila A; Hunter MS; Liang M; Yoon CH; Zhao J; Zatsepin NA; Abbey B; Sierecki E; Gambin Y; Stacey KJ; Darmanin C; Kobe B; Xu H; Ve T, 2021, 'MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography', ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 77, pp. C295 - C295, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000761714400289&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Journal articles | 2021
    Clabbers MTB; Holmes S; Muusse TW; Vajjhala PR; Thygesen SJ; Malde AK; Hunter DJB; Croll TI; Flueckiger L; Nanson JD; Rahaman MH; Aquila A; Hunter MS; Liang M; Yoon CH; Zhao J; Zatsepin NA; Abbey B; Sierecki E; Gambin Y; Stacey KJ; Darmanin C; Kobe B; Xu H; Ve T, 2021, 'MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography', Nature Communications, 12, http://dx.doi.org/10.1038/s41467-021-22590-6
    Journal articles | 2021
    Holmes S; Clabbers MTB; Muusse TW; Vajjhala PR; Thygesen SJ; Malde AK; Hunter DJB; Croll TI; Flueckiger L; Nanson JD; Rahaman MH; Aquila A; Hunter MS; Liang M; Yoon CH; Zhao J; Zatsepin NA; Abbey B; Sierecki E; Gambin Y; Stacey KJ; Darmanin C; Kobe B; Xu H; Ve T, 2021, 'MyD88 TIR domain higher-order assembly interactions revealed by serial femtosecond crystallography', ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES, 77, pp. C849 - C849, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000761714400826&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Journal articles | 2021
    Leitão ADG; Rudolffi-Soto P; Chappard A; Bhumkar A; Lau D; Hunter DJB; Gambin Y; Sierecki E, 2021, 'Selectivity of Lewy body protein interactions along the aggregation pathway of α-synuclein', Communications Biology, 4, http://dx.doi.org/10.1038/s42003-021-02624-x
    Journal articles | 2021
    McCann AJ; Lou J; Moustaqil M; Graus MS; Blum A; Fontaine F; Liu H; Luu W; Rudolffi-Soto P; Koopman P; Sierecki E; Gambin Y; Meunier FA; Liu Z; Hinde E; Francois M, 2021, 'A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity', Nucleic Acids Research, 49, pp. 10931 - 10955, http://dx.doi.org/10.1093/nar/gkab820
    Journal articles | 2021
    McMahon KA; Stroud DA; Gambin Y; Tillu VA; Bastiani M; Sierecki E; Polinkovsky M; Hall TE; Gomez GA; Wu Y; Parat MO; Martel N; Lo HP; Khanna KK; Alexandrov K; Daly R; Yap AS; Ryan MT; Parton RG, 2021, 'Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response', eLife, 10, http://dx.doi.org/10.7554/eLife.61407
    Journal articles | 2021
    Meyer B; Chiaravalli J; Gellenoncourt S; Brownridge P; Bryne DP; Daly LA; Grauslys A; Walter M; Agou F; Chakrabarti LA; Craik CS; Eyers CE; Eyers PA; Gambin Y; Jones AR; Sierecki E; Verdin E; Vignuzzi M; Emmott E, 2021, 'Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential', Nature Communications, 12, http://dx.doi.org/10.1038/s41467-021-25796-w
    Journal articles | 2021
    Moustaqil M; Ollivier E; Chiu HP; Van Tol S; Rudolffi-Soto P; Stevens C; Bhumkar A; Hunter DJB; Freiberg AN; Jacques D; Lee B; Sierecki E; Gambin Y, 2021, 'SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species', Emerging Microbes and Infections, 10, pp. 178 - 195, http://dx.doi.org/10.1080/22221751.2020.1870414
    Journal articles | 2021
    Shanmugam N; Baker MODG; Sanz-Hernandez M; Sierecki E; Gambin Y; Steain M; Pham CLL; Sunde M, 2021, 'Herpes simplex virus encoded ICP6 protein forms functional amyloid assemblies with necroptosis-associated host proteins', Biophysical Chemistry, 269, pp. 106524, http://dx.doi.org/10.1016/j.bpc.2020.106524
    Journal articles | 2020
    Moustaqil M; Gambin Y; Sierecki E, 2020, 'Biophysical techniques for target validation and drug discovery in transcription-targeted therapy', International Journal of Molecular Sciences, 21, http://dx.doi.org/10.3390/ijms21072301
    Journal articles | 2020
    Moustaqil M; Ollivier E; Chiu H-P; Van Tol S; Rudolffi-Soto P; Stevens C; Bhumkar A; Hunter DJB; Freiberg A; Jacques D; Lee B; Sierecki E; Gambin Y, 2020, 'SARS-CoV-2 proteases cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species and the search for reservoir hosts', , http://dx.doi.org/10.1101/2020.06.05.135699
    Journal articles | 2020
    Sierecki E; Gambin Y, 2020, 'Editorial', Seminars in Cell and Developmental Biology, 99, pp. 1 - 2, http://dx.doi.org/10.1016/j.semcdb.2019.11.007
    Journal articles | 2020
    Sierecki E, 2020, 'The Mediator complex and the role of protein-protein interactions in the gene regulation machinery', Seminars in Cell and Developmental Biology, 99, pp. 20 - 30, http://dx.doi.org/10.1016/j.semcdb.2018.08.006
    Journal articles | 2020
    Steain M; Baker MODG; Pham CLL; Shanmugam N; Gambin Y; Sierecki E; McSharry BP; Avdic S; Slobedman B; Sunde M; Abendroth A, 2020, 'Varicella zoster virus encodes a viral decoy RHIM to inhibit cell death', PLoS Pathogens, 16, pp. e1008473, http://dx.doi.org/10.1371/journal.ppat.1008473
    Journal articles | 2019
    Brown JWP; Bauer A; Polinkovsky ME; Bhumkar A; Hunter DJB; Gaus K; Sierecki E; Gambin Y, 2019, 'Single-molecule detection on a portable 3D-printed microscope', Nature Communications, 10, http://dx.doi.org/10.1038/s41467-019-13617-0
    Journal articles | 2019
    McMahon KA; Wu Y; Gambin Y; Sierecki E; Tillu VA; Hall T; Martel N; Okano S; Moradi SV; Ruelcke JE; Ferguson C; Yap AS; Alexandrov K; Hill MM; Parton RG, 2019, 'Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis', Nature Communications, 10, http://dx.doi.org/10.1038/s41467-019-11111-1
    Journal articles | 2019
    Monserrat-Martinez A; Gambin Y; Sierecki E, 2019, 'Thinking outside the bug: Molecular targets and strategies to overcome antibiotic resistance', International Journal of Molecular Sciences, 20, http://dx.doi.org/10.3390/ijms20061255
    Journal articles | 2019
    Overman J; Fontaine F; Sears JW; Moustaqil M; Huang L; Meurer M; Chiang IK; Lesieur E; Patel J; Zuegg J; Pasquier E; Sierecki E; Gambin Y; Hamdan M; Khosrotehrani K; Andelfinger G; Bischoff J; Francois M, 2019, 'R-propranolol is a small molecule inhibitor of the SOX18 transcription factor in a rare vascular syndrome and hemangioma', eLife, 8, http://dx.doi.org/10.7554/eLife.43026
    Journal articles | 2019
    Pang SS; Bayly-Jones C; Radjainia M; Spicer BA; Law RHP; Hodel AW; Parsons ES; Ekkel SM; Conroy PJ; Ramm G; Venugopal H; Bird PI; Hoogenboom BW; Voskoboinik I; Gambin Y; Sierecki E; Dunstone MA; Whisstock JC, 2019, 'The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1', Nature Communications, 10, http://dx.doi.org/10.1038/s41467-019-12279-2
    Journal articles | 2019
    Pham CLL; Shanmugam N; Strange M; O'Carroll A; Brown JWP; Sierecki E; Gambin Y; Steain M; Sunde M, 2019, 'Viral M45 and necroptosis-associated proteins form heteromeric amyloid assemblies', EMBO Reports, 20, pp. embr201846518, http://dx.doi.org/10.15252/embr.201846518
    Journal articles | 2019
    Sierecki E, 2019, 'A Novel Split Reporter Uncages New Possibilities', ACS Central Science, 5, pp. 1744 - 1746, http://dx.doi.org/10.1021/acscentsci.9b01051
    Journal articles | 2018
    Ariotti N; Rae J; Giles N; Martel N; Sierecki E; Gambin Y; Hall TE; Parton RG, 2018, 'Ultrastructural localisation of protein interactions using conditionally stable nanobodies', PLoS Biology, 16, pp. e2005473, http://dx.doi.org/10.1371/journal.pbio.2005473
    Journal articles | 2018
    Fontaine FR; Goodall S; Prokop JW; Howard CB; Moustaqil M; Kumble S; Rasicci DT; Osborne GW; Gambin Y; Sierecki E; Jones ML; Zuegg J; Mahler S; Francois M, 2018, 'Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach', mAbs, 10, pp. 596 - 606, http://dx.doi.org/10.1080/19420862.2018.1451288
    Journal articles | 2018
    Gambin Y; Giles N; O'Carroll A; Polinkovsky M; Hunter D; Sierecki E, 2018, 'Single-Molecule Fluorescence Reveals the Oligomerization and Folding Steps Driving the Prion-like Behavior of ASC', Journal of Molecular Biology, 430, pp. 491 - 508, http://dx.doi.org/10.1016/j.jmb.2017.12.013
    Journal articles | 2018
    Gambin Y; Giles N; O'Carroll A; Polinkovsky ME; Hunter DJB; Sierecki E, 2018, 'Corrigendum to “Single-Molecule Fluorescence Reveals the Oligomerisation and Folding Steps Driving the Prion-like Behaviour of ASC” [J. Mol. Biol. 430 (4) (February 16, 2018) 491–508] (S0022283617305946) (10.1016/j.jmb.2017.12.013))', Journal of Molecular Biology, 430, pp. 1263, http://dx.doi.org/10.1016/j.jmb.2018.02.011
    Journal articles | 2018
    Healey RD; Wojciechowski JP; Monserrat-Martinez A; Tan SL; Marquis CP; Sierecki E; Gambin Y; Finch AM; Thordarson P, 2018, 'Design, Synthesis, and Evaluation of N- and C-Terminal Protein Bioconjugates as G Protein-Coupled Receptor Agonists', Bioconjugate Chemistry, 29, pp. 403 - 409, http://dx.doi.org/10.1021/acs.bioconjchem.7b00716
    Journal articles | 2018
    Hunter DJB; Bhumkar A; Giles N; Sierecki E; Gambin Y, 2018, 'Unexpected instabilities explain batch-to-batch variability in cell-free protein expression systems', Biotechnology and Bioengineering, 115, pp. 1904 - 1914, http://dx.doi.org/10.1002/bit.26604
    Journal articles | 2018
    Hussein WM; Choi PM; Zhang C; Sierecki E; Johnston W; Jia Z; Monteiro MJ; Skwarczynski M; Gambin Y; Toth I, 2018, 'Investigating the affinity of poly tert-butyl acrylate toward Toll-Like Receptor 2', AIMS ALLERGY AND IMMUNOLOGY, 2, pp. 141 - 147, http://dx.doi.org/10.3934/Allergy.2018.3.141
    Journal articles | 2018
    Jung WR; Sierecki E; Bastiani M; O'Carroll A; Alexandrov K; Rae J; Johnston W; Hunter DJB; Ferguson C; Gambin Y; Ariotti N; Parton RG, 2018, 'Cell-free formation and interactome analysis of caveolae', Journal of Cell Biology, 217, pp. 2141 - 2165, http://dx.doi.org/10.1083/jcb.201707004
    Journal articles | 2018
    Leitao A; Bhumkar A; Hunter DJB; Gambin Y; Sierecki E, 2018, 'Unveiling a selective mechanism for the inhibition of α-synuclein aggregation by β-synuclein', International Journal of Molecular Sciences, 19, pp. 334, http://dx.doi.org/10.3390/ijms19020334
    Journal articles | 2018
    Moustaqil M; Fontaine F; Overman J; McCann A; Bailey TL; Soto PR; Bhumkar A; Giles N; Hunter DJB; Gambin Y; Francois M; Sierecki E, 2018, 'Homodimerization regulates an endothelial specific signature of the SOX18 transcription factor', Nucleic Acids Research, 46, pp. 11381 - 11395, http://dx.doi.org/10.1093/nar/gky897
    Journal articles | 2018
    O'Carroll A; Chauvin B; Brown JWP; Meagher A; Coyle J; Schill J; Bhumkhar A; Hunter DJB; Ve T; Kobe B; Sierecki E; Gambin Y, 2018, 'Pathological mutations differentially affect the self-assembly and polymerisation of the innate immune system signalling adaptor molecule MyD88', BMC Biology, 16, http://dx.doi.org/10.1186/s12915-018-0611-7
    Journal articles | 2018
    Steinbeck J; Ross IL; Rothnagel R; Gäbelein P; Schulze S; Giles N; Ali R; Drysdale R; Sierecki E; Gambin Y; Stahlberg H; Takahashi Y; Hippler M; Hankamer B, 2018, 'Structure of a PSI-LHCI-cyt b6f supercomplex in Chlamydomonas reinhardtii promoting cyclic electron flow under anaerobic conditions', Proceedings of the National Academy of Sciences of the United States of America, 115, pp. 10517 - 10522, http://dx.doi.org/10.1073/pnas.1809973115
    Journal articles | 2017
    Fontaine F; Overman J; Moustaqil M; Mamidyala S; Salim A; Narasimhan K; Prokoph N; Robertson AAB; Lua L; Alexandrov K; Koopman P; Capon RJ; Sierecki E; Gambin Y; Jauch R; Cooper MA; Zuegg J; Francois M, 2017, 'Small-Molecule Inhibitors of the SOX18 Transcription Factor', Cell Chemical Biology, 24, pp. 346 - 359, http://dx.doi.org/10.1016/j.chembiol.2017.01.003
    Journal articles | 2017
    Moustaqil M; Bhumkar A; Gonzalez L; Raoul L; Hunter DJB; Carrive P; Sierecki E; Gambin Y, 2017, 'A split-luciferase reporter recognizing GFP and mCherry tags to facilitate studies of protein–protein interactions', International Journal of Molecular Sciences, 18, http://dx.doi.org/10.3390/ijms18122681
    Journal articles | 2017
    Moustaqil MD; Fontaine F; Overman J; Giles ND; Bhumkar AD; O'Caroll AD; Francois M; Gambin YD; Sierecki ED, 2017, 'SOX18 transcription factor interactome: Protein‐Protein interaction a new road for anti‐cancer drug discovery', The FASEB Journal, 31, http://dx.doi.org/10.1096/fasebj.31.1_supplement.908.1
    Journal articles | 2017
    Overman J; Fontaine F; Moustaqil M; Mittal D; Sierecki E; Sacilotto N; Zuegg J; Robertson AAB; Holmes K; Salim AA; Mamidyala S; Butler MS; Robinson AS; Lesieur E; Johnston W; Alexandrov K; Black BL; Hogan BM; De Val S; Capon RJ; Carroll JS; Bailey TL; Koopman P; Jauch R; Smyth MJ; Cooper MA; Gambin Y; Francois M, 2017, 'Pharmacological targeting of the transcription factor SOX18 delays breast cancer in mice', eLife, 6, pp. e21221, http://dx.doi.org/10.7554/eLife.21221
    Journal articles | 2017
    Ve T; Vajjhala PR; Hedger A; Croll T; Dimaio F; Horsefield S; Yu X; Lavrencic P; Hassan Z; Morgan GP; Mansell A; Mobli M; O'Carroll A; Chauvin B; Gambin Y; Sierecki E; Landsberg MJ; Stacey KJ; Egelman EH; Kobe B, 2017, 'Structural basis of TIR-domain-assembly formation in MAL- and MyD88-dependent TLR4 signaling', Nature Structural and Molecular Biology, 24, pp. 743 - 751, http://dx.doi.org/10.1038/nsmb.3444
    Journal articles | 2016
    Chai YJ; Sierecki E; Tomatis VM; Gormal RS; Giles N; Morrow IC; Xia D; Götz J; Parton RG; Collins BM; Gambin Y; Meunier FA, 2016, 'Munc18-1 is a molecular chaperone for α-synuclein, controlling its self-replicating aggregation', Journal of Cell Biology, 214, http://dx.doi.org/10.1083/jcb.201512016
    Journal articles | 2016
    Gambin Y; Polinkovsky M; Francois B; Giles N; Bhumkar A; Sierecki E, 2016, 'Confocal Spectroscopy to Study Dimerization, Oligomerization and Aggregation of Proteins: A Practical Guide', International journal of molecular sciences, 17, http://dx.doi.org/10.3390/ijms17050655
    Journal articles | 2016
    Hussein WM; Choi PM; Zhang C; Su M; Sierecki E; Johnston W; Fagan V; Alexandrov K; Skwarczynski M; Gambin Y; Toth I; Simerska P, 2016, 'Evaluation of lipopeptides as toll-like receptor 2 ligands', Current Drug Delivery, 13, http://dx.doi.org/10.2174/1567201813666160804114107
    Journal articles | 2016
    Sierecki E; Giles N; Bowden Q; Polinkovsky ME; Steinbeck J; Arrioti N; Rahman D; Bhumkar A; Nicovich PR; Ross I, 2016, 'Nanomolar oligomerization and selective co-aggregation of α-synuclein pathogenic mutants revealed by single-molecule fluorescence', , http://dx.doi.org/10.1038/srep37630
    Journal articles | 2014
    Brooks AJ; Dai W; O'Mara ML; Abankwa D; Chhabra Y; Pelekanos RA; Gardon O; Tunny KA; Blucher KM; Morton CJ; Parker MW; Sierecki E; Gambin Y; Gomez GA; Alexandrov K; Wilson IA; Doxastakis M; Mark AE; Waters MJ, 2014, 'Mechanism of activation of protein kinase JAK2 by the growth hormone receptor', Science, 344, http://dx.doi.org/10.1126/science.1249783
    Journal articles | 2014
    Gambin Y; Ariotti N; McMahon KA; Bastiani M; Sierecki E; Kovtun O; Polinkovsky ME; Magenau A; Jung W; Okano S; Zhou Y; Leneva N; Mureev S; Johnston W; Gaus K; Hancock JF; Collins BM; Alexandrov K; Parton RG, 2014, 'Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae', eLife, 3, pp. e01434, http://dx.doi.org/10.7554/eLife.01434
    Journal articles | 2014
    Gambin Y; Ariotti N; McMahon KA; Bastiani M; Sierecki E; Kovtun O; Polinkovsky ME; Magenau A; Jung WR; Okano S; Zhou Y; Leneva N; Mureev S; Johnston W; Gaus K; Hancock JF; Collins BM; Alexandrov K; Parton RG, 2014, 'Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae', eLife, 2014, pp. e01434, http://dx.doi.org/10.7554/eLife.01434.001
    Journal articles | 2014
    Gambin Y; Sierecki E; Polinkovsky M; Alexandrov K; Parton R, 2014, 'Single molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae (602.1)', The FASEB Journal, 28, http://dx.doi.org/10.1096/fasebj.28.1_supplement.602.1
    Journal articles | 2014
    Han SP; Gambin Y; Gomez GA; Verma S; Giles N; Michael M; Wu SK; Guo Z; Johnston W; Sierecki E; Parton RG; Alexandrov K; Yap AS, 2014, 'Cortactin scaffolds Arp2/3 and WAVE2 at the epithelial zonula adherens', Journal of Biological Chemistry, 289, pp. 7764 - 7775, http://dx.doi.org/10.1074/jbc.M113.544478
    Journal articles | 2014
    Martin S; Papadopulos A; Tomatis VM; Sierecki E; Malintan NT; Gormal RS; Giles N; Johnston WA; Alexandrov K; Gambin Y; Collins BM; Meunier FA, 2014, 'Increased polyubiquitination and proteasomal degradation of a Munc18-1 disease-linked mutant causes temperature-sensitive defect in exocytosis', Cell Reports, 9, pp. 206 - 218, http://dx.doi.org/10.1016/j.celrep.2014.08.059
    Journal articles | 2014
    Reyes G; Niederst M; Cohen-Katsenelson K; Stender JD; Kunkel MT; Chen M; Brognard J; Sierecki E; Gao T; Nowak DG; Trotman LC; Glass CK; Newton AC, 2014, 'Pleckstrin homology domain leucine-rich repeat protein phosphatases set the amplitude of receptor tyrosine kinase output', Proceedings of the National Academy of Sciences of the United States of America, 111, pp. E3957 - E3965, http://dx.doi.org/10.1073/pnas.1404221111
    Journal articles | 2014
    Sierecki E; Newton AC, 2014, 'Biochemical characterization of the phosphatase domain of the tumor suppressor PH domain leucine-rich repeat protein phosphatase', Biochemistry, 53, pp. 3971 - 3981, http://dx.doi.org/10.1021/bi500428j
    Journal articles | 2014
    Sierecki E; Stevers LM; Giles N; Polinkovsky ME; Moustaqil M; Mureev S; Johnston WA; Dahmer-Heath M; Skalamera D; Gonda TJ; Gabrielli B; Collins BM; Alexandrov K; Gambin Y, 2014, 'Rapid mapping of interactions between human SNX-BAR proteins measured in vitro by alphascreen and single-molecule spectroscopy', Molecular and Cellular Proteomics, 13, pp. 2233 - 2245, http://dx.doi.org/10.1074/mcp.M113.037275
    Journal articles | 2014
    Wang P; Zhou Z; Hu A; Pontede Albuquerque C; Zhou Y; Hong L; Sierecki E; Ajiro M; Kruhlak M; Harris C; Guan KL; Zheng ZM; Newton AC; Sun P; Zhou H; Fu XD, 2014, 'Both decreased and increased SRPK1 levels promote cancer by interfering with PHLPP-mediated dephosphorylation of Akt', Molecular Cell, 54, pp. 378 - 391, http://dx.doi.org/10.1016/j.molcel.2014.03.007
    Journal articles | 2013
    Sierecki E; Giles N; Polinkovsky M; Moustaqil M; Alexandrov K; Gambin Y, 2013, 'A cell-free approach to accelerate the study of protein-protein interactions in vitro', Interface Focus, 3, http://dx.doi.org/10.1098/rsfs.2013.0018
    Journal articles | 2012
    Sinko W; Sierecki E; De Oliveira CAF; McCammon JA, 2012, 'Guide to virtual screening: Application to the akt phosphatase PHLPP', Methods in Molecular Biology, 819, pp. 561 - 573, http://dx.doi.org/10.1007/978-1-61779-465-0_33
    Journal articles | 2010
    Gambin Y; Reffay M; Sierecki E; Homblé F; Hodges RS; Gov NS; Taulier N; Urbach W, 2010, 'Variation of the lateral mobility of transmembrane peptides with hydrophobic mismatch', Journal of Physical Chemistry B, 114, pp. 3559 - 3566, http://dx.doi.org/10.1021/jp911354y
    Journal articles | 2010
    Sierecki E; Errasti G; Martens T; Royer J, 2010, 'Diastereoselective α-allylation of secondary amines', Tetrahedron, 66, pp. 10002 - 10007, http://dx.doi.org/10.1016/j.tet.2010.10.031
    Journal articles | 2010
    Sierecki E; Sinko W; McCammon JA; Newton AC, 2010, 'Discovery of small molecule inhibitors of the ph domain leucine-rich repeat protein phosphatase (PHLPP) by chemical and virtual screening', Journal of Medicinal Chemistry, 53, pp. 6899 - 6911, http://dx.doi.org/10.1021/jm100331d
    Journal articles | 2009
    Sierecki E; Newton AC, 2009, 'Modulation of PHLPP activity: screening for specific inhibitors', The FASEB Journal, 23, http://dx.doi.org/10.1096/fasebj.23.1_supplement.lb289
    Journal articles | 2007
    Brognard J; Sierecki E; Gao T; Newton AC, 2007, 'PHLPP and a Second Isoform, PHLPP2, Differentially Attenuate the Amplitude of Akt Signaling by Regulating Distinct Akt Isoforms', Molecular Cell, 25, pp. 917 - 931, http://dx.doi.org/10.1016/j.molcel.2007.02.017
    Journal articles | 2007
    Turcaud S; Sierecki E; Martens T; Royer J, 2007, 'Asymmetric α-alkynylation of piperidine via N-sulfinyliminium salts', Journal of Organic Chemistry, 72, pp. 4882 - 4885, http://dx.doi.org/10.1021/jo070631c
    Journal articles | 2007
    Turcaud S; Sierecki E; Martens T; Royer J, 2007, 'Asymmetric α‐Alkynylation of Piperidine via N‐Sulfinyliminium Salts.', ChemInform, 38, http://dx.doi.org/10.1002/chin.200749155
    Journal articles | 2006
    Gambin Y; Lopez-Esparza R; Reffay M; Sierecki E; Gov NS; Genest M; Hodges RS; Urbach W, 2006, 'Lateral mobility of proteins in liquid membranes revisited', Proceedings of the National Academy of Sciences of the United States of America, 103, pp. 2098 - 2102, http://dx.doi.org/10.1073/pnas.0511026103
    Journal articles | 2006
    Sierecki E; Turcaud S; Martens T; Royer J, 2006, 'Phosphorus-derived chiral auxiliaries for α-alkylation of secondary amines by anodic oxidation', Synthesis, pp. 3199 - 3208, http://dx.doi.org/10.1055/s-2006-950226
    Journal articles | 2005
    Turcaud S; Martens T; Sierecki E; Perard‐Viret J; Royer J, 2005, 'Anodic Oxidation of Chiral Sulfinylamines: A New Route to Highly Diastereoselective α‐Alkylation of Piperidine.', ChemInform, 36, http://dx.doi.org/10.1002/chin.200547134
    Journal articles | 2005
    Turcaud S; Martens T; Sierecki E; Pérard-Viret J; Royer J, 2005, 'Anodic oxidation of chiral sulfinylamines: A new route to highly diastereoselective α-alkylation of piperidine', Tetrahedron Letters, 46, pp. 5131 - 5134, http://dx.doi.org/10.1016/j.tetlet.2005.05.123
  • Preprints | 2024
    Houx J; Copie T; Gambin Y; Sierecki E, 2024, Quantification of nanocondensates formation at the single molecule level, , http://dx.doi.org/10.1101/2024.05.05.592604
    Preprints | 2024
    Lau D; Tang Y; Kenche V; Copie T; Kempe D; Jary E; Graves NJ; Biro M; Masters CL; Dzamko N; Gambin Y; Sierecki E, 2024, Single molecule fingerprinting reveals different growth mechanisms in seed amplification assays for different polymorphs of αSynuclein fibrils, , http://dx.doi.org/10.1101/2024.03.05.583619
    Preprints | 2024
    Uckelmann M; Levina V; Taveneau C; Han Ng X; Pandey V; Martinez J; Mendiratta S; Houx J; Boudes M; Venugopal H; Trépout S; Zhang Q; Flanigan S; Li M; Sierecki E; Gambin Y; Das PP; Bell O; de Marco A; Davidovich C, 2024, Dynamic PRC1-CBX8 stabilizes a porous structure of chromatin condensates., , http://dx.doi.org/10.1101/2023.05.08.539931
    Preprints | 2023
    Dickson CF; Hertel S; Ruan J; Ariotti N; Tuckwell A; Li N; Al-Izzi SC; Sierecki E; Gambin Y; Morris RG; Towers GJ; Böcking T; Jacques DA, 2023, Karyopherin mimicry explains how the HIV capsid penetrates nuclear pores, , http://dx.doi.org/10.1101/2023.03.23.534032
    Preprints | 2021
    Chiang IK-N; Luu W; Jiang K; Kirschnick N; Moustaqil M; Davidson T; Lesieur E; Skoczylas R; Kouskoff V; Kazenwadel J; Arriola-Martinez L; Sierecki E; Gambin Y; Alitalo K; Kiefer F; Harvey N; Francois M, 2021, The blood vasculature instructs lymphatics patterning in a SOX7 dependent manner, http://dx.doi.org/10.1101/2021.07.02.450967
    Preprints | 2021
    Lau D; Magnan C; Hill K; Cooper A; Gambin Y; Sierecki E, 2021, Single molecule fingerprinting reveals different amplification properties of α-synuclein oligomers and preformed fibrils in seeding assay, http://dx.doi.org/10.1101/2021.08.09.455607
    Preprints | 2021
    Leitão A; Soto PR; Chappard A; Bhumkar A; Hunter D; Sierecki E; Gambin Y, 2021, Selectivity of Protein Interactions along the Aggregation Pathway of α-Synuclein, , http://dx.doi.org/10.1101/2021.01.29.428744
    Preprints | 2020
    Lau D; Walsh J; Dickson C; Tuckwell A; Stear J; Hunter D; Bhumkar A; Shah V; Turville S; Sierecki E; Gambin Y; Böcking T; Jacques D, 2020, Rapid HIV-1 capsid interaction screening using fluorescence fluctuation spectroscopy, http://dx.doi.org/10.1101/2020.11.13.382242
    Preprints | 2020
    McCann A; Lou J; Moustaqil M; Blum A; Fontaine F; Liu H; Luu W; Koopman P; Sierecki E; Gambin Y; Meunier F; Liu Z; Hinde E; Francois M, 2020, A dominant-negative SOX18 mutant disrupts multiple regulatory layers essential to transcription factor activity, http://dx.doi.org/10.1101/2020.11.11.378968
    Preprints | 2020
    McMahon K-A; Stroud D; Gambin Y; Tillu V; Bastiani M; Sierecki E; Polinkovsky M; Hall T; Gomez G; Wu Y; Parat M-O; Martel N; Lo H; Khanna KK; Alexandrov K; Daly R; Yap A; Ryan M; Parton R, 2020, Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response, http://dx.doi.org/10.1101/2020.07.26.222158
    Preprints | 2020
    Meyer B; Chiaravalli J; Gellenoncourt S; Brownridge P; Bryne D; Daly L; Grauslys A; Walter M; Agou F; Chakrabarti L; Chakrabarti L; Craik C; Eyers C; Eyers P; Gambin Y; Jones A; Sierecki E; Verdin E; Vignuzzi M; Emmott E, 2020, Characterisation of protease activity during SARS-CoV-2 infection identifies novel viral cleavage sites and cellular targets with therapeutic potential, http://dx.doi.org/10.1101/2020.09.16.297945
    Preprints | 2020
    Steain M; Baker MODG; Pham CLL; Gambin Y; Sierecki E; McSharry B; Avdic S; Slobedman B; Sunde M; Abendroth A, 2020, Varicella zoster virus encodes a viral decoy RHIM to inhibit cell death, http://dx.doi.org/10.1101/2020.03.12.988659
    Conference Abstracts | 2020
    Sunde M; Pham CLL; Shanmugam N; Baker MODG; Steain M; O'Carroll A; Brown JW; Sierecki E; Gambin Y, 2020, 'Competing Interactions between Viral RHIM Amyloid-forming Proteins and Host Functional Amyloid Structures Modulate the Cellular Response to Infection', in Biophysical Journal, Elsevier, CA, San Diego, Vol. 118, pp. 180a - 181a, presented at 64th Annual Meeting of the Biophysical-Society, CA, San Diego, 15 February 2020 - 19 February 2020, http://dx.doi.org/10.1016/j.bpj.2019.11.1103
    Conference Abstracts | 2019
    Gambin Y; Monserrat-Martinez A; Sierecki E, 2019, 'ENTEROPATHOGENIC E. COLI HIJACKS PROGRAMMED HOST-CELL DEATH PATHWAYS BY INTERFERING WITH THE HIGHER ORDER OLIGOMERIZATION OF IMMUNE SYSTEM PROTEINS', in PROTEIN SCIENCE, WILEY, WA, Seattle, Vol. 28, pp. 131 - 132, presented at 33rd Annual Symposium of the Protein-Society, WA, Seattle, 30 June 2019 - 03 July 2019, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000488134600233&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2019
    Monserrat-Martinez A; Redpath G; Govendir MA; O'Carroll A; Murillo ADA; Sierecki E; Gambin Y, 2019, 'Enteropathogenic E. coli Hijacks Programmed Host-Cell Death Pathways by Interfering with the Higher Order Oligomerization of Immune System Proteins', in FASEB JOURNAL, WILEY, FL, Orlando, presented at Experimental Biology Meeting (EB), FL, Orlando, 06 April 2019 - 09 April 2019, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000495951205245&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Preprints | 2019
    Pang SS; Bayly-Jones C; Radjainia M; Spicer B; Law RHP; Hodel A; Ekkel S; Conroy P; Ramm G; Venugopal H; Bird P; Hoogenboom B; Voskoboinik I; Gambin Y; Sierecki E; Dunstone M; Whisstock J, 2019, The structure of Macrophage Expressed Gene-1, a phagolysosome immune effector that is activated upon acidification, http://dx.doi.org/10.1101/580712
    Conference Abstracts | 2019
    Sierecki E; Leitao A; Brown J; Chappard A; Gambin Y, 2019, 'MODULATION OF INTERACTOME BY PROTEIN SELF-ASSEMBLY: THE CASE OF ALPHA-SYNUCLEIN', in PROTEIN SCIENCE, WILEY, WA, Seattle, Vol. 28, pp. 132 - 132, presented at 33rd Annual Symposium of the Protein-Society, WA, Seattle, 30 June 2019 - 03 July 2019, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000488134600234&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Preprints | 2018
    O’Carroll A; Chauvin B; Brown J; Meagher A; Coyle J; Hunter D; Bhumkhar A; Ve T; Kobe B; Sierecki E; Gambin Y, 2018, Effects of Pathological Mutations on the Prion-Like Polymerisation of MyD88, , http://dx.doi.org/10.1101/351726
    Preprints | 2018
    Pham C; Strange M; O’ Carroll A; Shanmugam N; Sierecki E; Gambin Y; Steain M; Sunde M, 2018, The RHIM within the M45 protein from murine cytomegalovirus forms heteromeric amyloid fibrils with RIPK1 and RIPK3, , http://dx.doi.org/10.1101/324590
    Conference Abstracts | 2018
    Sierecki E; Brown J; Leitao A; Chappard A; Gambin Y, 2018, 'The Shifting Interactome of Alpha-Synuclein along its Aggregation Pathway', in PROTEIN SCIENCE, WILEY, MA, Boston, Vol. 27, pp. 73 - 73, presented at 32nd Annual Symposium of the Protein-Society, MA, Boston, 09 July 2018 - 12 July 2018, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000450682700119&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Abstracts | 2017
    O'Carroll A; Ve T; Moustaqil M; Giles N; Bhumkar A; Hunter D; Kobe B; Sierecki E; Gambin Y, 2017, 'Prion - like protein polymerisation underlies signal transduction in innate immunity: The emergence of a universal mechanism ?', in FASEB JOURNAL, WILEY, IL, Chicago, Vol. 31, presented at Experimental Biology (EB) Annual Meeting, IL, Chicago, 22 April 2017 - 26 April 2017, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000405461402427&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Bhumkar A; Gambin Y; Leitao A; Bowden Q; Giles N; Francois B; Hunter D; Boecking T; Sierecki E, 2016, 'What pathological mutants tell us about synuclein aggregation pathways', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 02 April 2016 - 06 April 2016, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444003009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Bhumkar A; Gambin Y; Leitao A; Bowden Q; Giles N; Francois B; Hunter D; Boecking T; Sierecki E, 2016, 'What pathological mutants tell us about synuclein aggregation pathways', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, San Diego, CA, presented at Experimental Biology Meeting, San Diego, CA, 02 April 2016 - 06 April 2016, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444005434&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Brooks AJ; O'Mara ML; Dai W; Abankwa D; Chhabra Y; Tunny KA; Parker MW; Sierecki E; Gambi Y; Gomez GA; Haxholm GW; Nikolajsen LF; Doxastakis M; Mark AE; Waters MJ, 2016, 'Mechanism of JAK2 Activation by the Archetype Class I Cytokine Receptor, the Growth Hormone Receptor', in BIOPHYSICAL JOURNAL, CELL PRESS, CA, Los Angeles, pp. 31A - 31A, presented at 60th Annual Meeting of the Biophysical-Society, CA, Los Angeles, 27 February 2016 - 02 March 2016, http://dx.doi.org/10.1016/j.bpj.2015.11.233
    Conference Papers | 2016
    Gambin Y; Sierecki E; Jung W; Ariotti N; Giles N; Bastiani M; Parton R, 2016, 'Redefining caveolae as signalling platforms: specificity of interactions on endocytic caveolae in response to stress', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 02 April 2016 - 06 April 2016, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444003550&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Gambin Y; Sierecki E; Jung W; Ariotti N; Giles N; Bastiani M; Parton R, 2016, 'Redefining caveolae as signalling platforms: specificity of interactions on endocytic caveolae in response to stress', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, San Diego, CA, presented at Experimental Biology Meeting, San Diego, CA, 02 April 2016 - 06 April 2016, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444006522&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Giles N; McCann A; Moustaqil M; Overman J; Fontaine F; Francois M; Sierecki E; Gambin Y, 2016, 'Tracking Dynamics of Vascular Development in Time and Space using Sox18 Dimer split-Fluorescent Protein Biosensors', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 02 April 2016 - 06 April 2016, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444003324&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Giles N; McCann A; Moustaqil M; Overman J; Fontaine F; Francois M; Sierecki E; Gambin Y, 2016, 'Tracking Dynamics of Vascular Development in Time and Space using Sox18 Dimer split-Fluorescent Protein Biosensors', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, San Diego, CA, presented at Experimental Biology Meeting, San Diego, CA, 02 April 2016 - 06 April 2016, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444006122&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Moustaqil M; Overman J; Fontaine F; Bailey T; Francois M; Gambin Y; Sierecki E, 2016, 'Establishing a transcription factors protein network for lymphangiogenesis: identification of binding domains, impact of pathological mutations and perturbations by small molecules', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 02 April 2016 - 06 April 2016, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444003094&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    Moustaqil M; Overman J; Fontaine F; Bailey T; Francois M; Gambin Y; Sierecki E, 2016, 'Establishing a transcription factors protein network for lymphangiogenesis: identification of binding domains, impact of pathological mutations and perturbations by small molecules', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, San Diego, CA, presented at Experimental Biology Meeting, San Diego, CA, 02 April 2016 - 06 April 2016, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444005311&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    O'Carroll A; Ve T; Lau D; Giles N; Moustaqil M; Bhumkar A; Hunter D; Kobe B; Boecking T; Sierecki E; Gambin Y, 2016, 'Prion - like protein polymerisation underlies signal transduction in innate immunity: the emergence of a universal mechanism ?', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 02 April 2016 - 06 April 2016, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444003010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2016
    O'Carroll A; Ve T; Lau D; Giles N; Moustaqil M; Bhumkar A; Hunter D; Kobe B; Boecking T; Sierecki E; Gambin Y, 2016, 'Prion - like protein polymerisation underlies signal transduction in innate immunity: the emergence of a universal mechanism ?', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, San Diego, CA, presented at Experimental Biology Meeting, San Diego, CA, 02 April 2016 - 06 April 2016, http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000406444005435&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2015
    Brooks A; Dai W; O'Mara ML; Abankwa D; Chhabra Y; Pelekanos RA; Gardon O; Tunny KA; Blucher KM; Morton CJ; Parker MW; Sierecki E; Gambin Y; Gomez GA; Alexandrov K; Wilson IA; Doxastakis M; Mark AE; Waters MJ, 2015, 'Going downstream - how does GH binding activate JAK2', in CLINICAL ENDOCRINOLOGY, WILEY, AUSTRALIA, Adelaide, pp. 16 - 16, presented at Annual Scientific Meeting of the Endocrine-Society-of-Australia, AUSTRALIA, Adelaide, 23 August 2015 - 26 August 2015, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000372310400043&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2014
    Brooks AJ; Chhabra Y; Abankwa D; O'Mara M; Dai W; Gardon O; Tunny KA; Blucher KM; Morton CJ; Parker MW; Sierecki E; Gambin Y; Gomez GA; Alexandrov KK; Doxastakis M; Mark AE; Waters MJ, 2014, 'A new cytokine receptor activation paradigm: Activation of JAK2 by the growth hormone receptor', in CYTOKINE, ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, AUSTRALIA, Melbourne, pp. 22 - 22, presented at 2nd Annual Meeting of the International-Cytokine-and-Interferon-Society (ICIS), AUSTRALIA, Melbourne, 26 October 2014 - 29 October 2014, http://dx.doi.org/10.1016/j.cyto.2014.07.227
    Conference Papers | 2014
    Giles N; Sierecki E; Polinkovsky M; Schroder K; Alexandrov K; Gambin Y, 2014, 'A novel approach to investigate the assembly of the NOD-like receptor inflammasomes', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, CA, San Diego, presented at Experimental Biology Meeting, CA, San Diego, 26 April 2014 - 30 April 2014, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000346646700166&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2014
    Polinkovsky M; Sierecki E; Giles N; Alexandrov K; Gambin Y, 2014, 'Uncovering transcription factor binding pockets in the tail subdomain of the human mediator complex', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000346651004001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2014
    Sierecki E; Polinkovsky M; Giles N; Moustaqil M; Alexandrov K; Gambin Y, 2014, 'Probing the architecture of the Mediator complex', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000346651003484&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2012
    Sinko W; Sierecki E; Newton AC; McCammon JA, 2012, 'Virtual and chemical screening leads to the discovery of small molecule inhibitors of the Akt phosphatase PHLPP', in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, AMER CHEMICAL SOC, CA, San Diego, presented at 11th International Biorelated Polymer Symposium / 243rd National Spring Meeting of the American-Chemical-Society (ACS), CA, San Diego, 25 March 2012 - 29 March 2012, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000324475104598&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2010
    Gambin Y; Reffay M; Sierecki E; Homble F; Genest M; Hodges RS; Gov N; Taulier N; Urbach W, 2010, 'Modulation of the Lateral Mobility of Transmembrane Peptides with Hydrophobic Mismatch', in BIOPHYSICAL JOURNAL, CELL PRESS, pp. 224A - 225A, http://dx.doi.org/10.1016/j.bpj.2009.12.1215
    Conference Papers | 2010
    Sierecki E; Sinko W; Mc Cammon JA; Newton AC, 2010, 'Small molecule inhibitors of PHLPP determined by chemical and virtual screening', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000208675506488&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2008
    Sierecki E; Newton AC, 2008, 'Biochemical characterization of the PP2C domain of PHLPP', in FASEB JOURNAL, FEDERATION AMER SOC EXP BIOL, https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000208467808801&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=891bb5ab6ba270e68a29b250adbe88d1
    Conference Papers | 2007
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The role of a protein can be profoundly affected by its protein partners. Specific interactions can select outputs when a protein serves as hub between different pathways or serve as a switch between on and off forms. Because of this crucial role, loss or gain of interactions play an important role in disease. In my laboratory, we are  particularly interested in neurodegenerative diseases and in studying the aggregation processes in Parkinson’s disease .

α-synuclein aggregation & diagnostics of Parkinson’s disease and other synucleinopathies

Neurodegenerative diseases such as Parkinson’s disease (PD) are a prime example of gains of interactions leading to pathologies. α-Synuclein (Syn) aggregation is a hallmark of PD and other related disorders (such as multiple system atrophy (MSA) or dementia with Lewy Bodies (DLB)), called synucleinopathies. Syn aggregation is a promising biomarker for early detection and assessment of disease progression. It was recently shown that Syn seeds could be detected and amplified from biological fluids, allowing to discriminate PD patients even at a prodromal phase. We showed interactions with other proteins could modulate Syn aggregation. First, we examined the effect of pathological mutants, linked to early-onset PD, on aggregation propensity. These mutants had an increased tendency to aggregates compared to wild type. Some mutants did not co-aggregate with WT Syn suggesting a structural imprinting by the mutations (Sci Rep 2016). We demonstrated that β-synuclein (IJMS 2018) and Munc18 (J.Cell.Bio 2016) prevented Syn aggregation, acting as molecular chaperones. The study was extended to other proteins partners found in the neuronal protein inclusions. We observed that self-association drove partner selectivity with some proteins recognizing the oligomeric but not fibrillar form of Syn (Comms Bio, under revision).